Egfr Knockout cell line (MC3T3-E1 Subclone 14)

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location： Home > Products > KO Cell Lines > Egfr Knockout cell line (MC3T3-E1 Subclone 14)

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Egfr Knockout cell line (MC3T3-E1 Subclone 14) Order

Egfr Knockout cell line (MC3T3-E1 Subclone 14)
Catalog#
YKO-M008
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Egfr Knockout cell line (MC3T3-E1 Subclone 14)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Egfr Knockout cell line (MC3T3-E1 Subclone 14)
Species
Mouse
Gene Name
Egfr
Gene ID
13649
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Egfr gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC3T3-E1 Subclone 14 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Egfr gene knockout Egfr Knockout cell line (MC3T3-E1 Subclone 14) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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